In Situ Bioremediation of Trichloroethylene

In situ bioremediation of chlorinated solvents.

Chlorinated solvents and their natural transformation products are the most frequently observed groundwater contaminants in the United States. In situ bioremediation using anaerobic or aerobic co-metabolic processes is a promising means of cleaning up contaminated aquifers. Studies show that under natural conditions trichloroethylene can be anaerobically degraded to dichloroethylene, vinyl chlo...

Mathematical Modeling of Natural in Situ Bioremediation to Estimate Initial Contaminant Concentration Effect

A mathematical model has been simulated to describe natural in situ bioremediation of contaminated soil. The model equations consist of a system of three non-linear partial differential equations. Sensitivity analysis conducted by numerically solving them, has demonstrated the effect of initial contaminant concentration on the time and mechanism of remediation. The result of simulation indicate...

Mathematical modeling of bioremediation of trichloroethylene in aquifers

Trichloroethylene (TCE) is a very common contaminant of groundwater. It is used as an industrial solvent and is frequently poured into the soil. There exist bacteria that can degrade TCE. In contrast with most cases of bioremediation, the bacteria that degrade TCE do not use it as a carbon source. Instead the bacteria produce an enzyme to metabolize methane. This enzyme can degrade other organi...

Use of Starvation Promoters To Limit Growth and Select for Trichloroethylene and Phenol Transformation Activity in Recombinant Escherichia coli

The expression of much useful bacterial activity is facilitated by rapid growth. This coupling can create problems in bacterial fermentations and in situ bioremediation. In the latter process, for example, it necessitates addition of large amounts of nutrients to contaminated environments, such as aquifers. This approach, termed biostimulation, can be technically difficult. Moreover, the result...

Trichloroethylene Bioremediation Assessment of Phenol-Stimulated Hydroxylase Genes for a Functional Group-Specific Monitoring of Phenol

Stable carbon isotope fractionation during aerobic biodegradation of chlorinated ethenes.

Stable isotope analysis is recognized as a powerful tool for monitoring, assessing, and validating in-situ bioremediation processes. In this study, kinetic carbon isotope fractionation factors (epsilon) associated with the aerobic biodegradation of vinyl chloride (VC), cis-1,2-dichloroethylene (cDCE), and trichloroethylene (TCE) were examined. Of the three solvents, the largest fractionation ef...